Assess subpopulation heterogeneity in clathrin-coated pit invagination pathways

Ascertain whether distinct subpopulations of clathrin-coated pits contribute to the analyzed datasets and determine whether these subpopulations follow different invagination pathways and mechanisms during clathrin-mediated endocytosis.

Background

The authors’ consensus pathway is derived from pooled, high-resolution structural datasets across multiple techniques and cell lines. However, they note considerable variability within individual datasets, the existence of abortive events, and the possibility of different subpopulations that may not be captured by the averaged description.

Establishing whether heterogeneous subpopulations exist and characterizing their specific pathways and mechanisms would refine the consensus trajectory and could reveal multiple modes of coat invagination operating under different cellular contexts or conditions.