Origin of ResLog differences and potential alignment biases in LPP reconstructions

Investigate whether the observed ResLog intercept and resolution trend differences between laser phase plate and defocus datasets are spurious or originate from biases in particle alignment—potentially induced by structural symmetry—and develop methods to mitigate such effects in single‑particle reconstruction workflows.

Background

For apoferritin, ResLog plots show different intercepts between LPP and defocus datasets. The authors question whether these differences are statistical artifacts or arise from misalignment biases related to contrast and symmetry.

Clarifying the origin will guide algorithmic adjustments and determine where LPP provides advantages or requires specialized processing.