GenCellAgent: Generalizable, Training-Free Cellular Image Segmentation via Large Language Model Agents

Abstract: Cellular image segmentation is essential for quantitative biology yet remains difficult due to heterogeneous modalities, morphological variability, and limited annotations. We present GenCellAgent, a training-free multi-agent framework that orchestrates specialist segmenters and generalist vision-LLMs via a planner-executor-evaluator loop (choose tool $\rightarrow$ run $\rightarrow$ quality-check) with long-term memory. The system (i) automatically routes images to the best tool, (ii) adapts on the fly using a few reference images when imaging conditions differ from what a tool expects, (iii) supports text-guided segmentation of organelles not covered by existing models, and (iv) commits expert edits to memory, enabling self-evolution and personalized workflows. Across four cell-segmentation benchmarks, this routing yields a 15.7\% mean accuracy gain over state-of-the-art baselines. On endoplasmic reticulum and mitochondria from new datasets, GenCellAgent improves average IoU by 37.6\% over specialist models. It also segments novel objects such as the Golgi apparatus via iterative text-guided refinement, with light human correction further boosting performance. Together, these capabilities provide a practical path to robust, adaptable cellular image segmentation without retraining, while reducing annotation burden and matching user preferences.