Assessing ChatGPT-4’s ability to evaluate ORF coding potential

Determine whether ChatGPT-4 can precisely assess the protein-coding potential of individual open reading frames (ORFs) in DNA sequences.

Background

Identifying protein-coding regions in genomes relies on detecting open reading frames (ORFs) and evaluating their likelihood of encoding proteins. The review describes that ChatGPT-4 can list potential ORFs in viral sequences and, with chain-of-thought prompting, identify the longest ORFs, but it is unclear if it can accurately judge coding potential.

Establishing whether ChatGPT-4 can reliably assess ORF coding potential would clarify its utility in genome annotation tasks and delineate where expert validation or additional computational methods remain necessary.