Light and Sound Driven Wavefront Shaping and Imaging through Scattering Tissue

Abstract: Deep, high-resolution imaging is essential for unraveling biological complexity and advancing medical diagnostics, yet scattering fundamentally limits optical methods. Among the most promising approaches, photoacoustic imaging achieves penetration into deep tissue but with coarse resolution, while fluorescence provides subcellular detail but is confined to shallow depths. This depth-resolution trade-off remains a central barrier to biomedical imaging. To bridge this fundamental gap, we present a hybrid dual-modal strategy that combines the benefits of photoacoustic and fluorescence modalities. Our approach leverages hybrid opto-acoustic feedback for wavefront shaping and computational imaging through scattering media. By combining these complementary signals into a nonlinear feedback metric, we achieve robust optical focusing even under signal degradation. In particular, we show that photoacoustic-guided wavefront shaping inherently generates fluorescence that can be harvested for computational high-resolution imaging even within highly scattering biological tissues, thereby leveraging the complementary strengths of both modalities in a single framework. Proof-of-concept experiments demonstrate this synergistic approach, paving the way for optical imaging techniques that fully leverage the potential of such dual-modalities for large depth penetration and high resolution in complex biological tissues.