Intelligent Nano-Fingerprinting: An Efficient and Precise Approach for Liquid Biopsy

Abstract: Biological matrices are rich in information related to life processes, serving as invaluable media for assessing an individual's overall physiological status and its dynamic fluctuations, as well as crucial foundations for disease diagnosis. However, the inherent complexity of these matrices, coupled with our incomplete understanding of their full composition, presents significant challenges for comprehensive analysis and accurate diagnostic interpretation. The advent of single-molecule technologies has revolutionized biomedical research, enabling the direct observation of life processes at the molecular scale. We have proposed an Intelligent Nano-Fingerprinting strategy based on single-molecule nanopore technology, designed to capture the global molecular fingerprints of complex plasma matrices. Furthermore, we developed an intelligent algorithmic model capable of achieving precise classification of plasma samples. This approach is characterized by its simplicity, efficiency, and considerable potential for large-scale adoption and transferable applications.