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Automated Classification of Cell Shapes: A Comparative Evaluation of Shape Descriptors

Published 1 Nov 2024 in cs.CV and q-bio.QM | (2411.00561v1)

Abstract: This study addresses the challenge of classifying cell shapes from noisy contours, such as those obtained through cell instance segmentation of histological images. We assess the performance of various features for shape classification, including Elliptical Fourier Descriptors, curvature features, and lower dimensional representations. Using an annotated synthetic dataset of noisy contours, we identify the most suitable shape descriptors and apply them to a set of real images for qualitative analysis. Our aim is to provide a comprehensive evaluation of descriptors for classifying cell shapes, which can support cell type identification and tissue characterization-critical tasks in both biological research and histopathological assessments.

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References (23)
  1. E. Alizadeh, J. Castle, A. Quirk, C. D. Taylor, W. Xu, and A. Prasad, “Cellular morphological features are predictive markers of cancer cell state,” Computers in biology and medicine, vol. 126, p. 104044, 2020.
  2. P.-H. Wu, D. M. Gilkes, J. M. Phillip, A. Narkar, T. W.-T. Cheng, J. Marchand, M.-H. Lee, R. Li, and D. Wirtz, “Single-cell morphology encodes metastatic potential,” Science advances, vol. 6, no. 4, p. eaaw6938, 2020.
  3. H. Zeng, “What is a cell type and how to define it?” Cell, vol. 185, no. 15, pp. 2739–2755, 2022.
  4. A. Dance, “What is a cell type, really? the quest to categorize life’s myriad forms.” Nature, vol. 633, no. 8031, pp. 754–756, 2024.
  5. L. Corain, E. Grisan, J.-M. Graïc, R. Carvajal-Schiaffino, B. Cozzi, and A. Peruffo, “Multi-aspect testing and ranking inference to quantify dimorphism in the cytoarchitecture of cerebellum of male, female and intersex individuals: a model applied to bovine brains,” Brain Structure and Function, vol. 225, no. 9, pp. 2669–2688, 2020.
  6. K. Amunts and K. Zilles, “Architectonic mapping of the human brain beyond brodmann,” Neuron, vol. 88, no. 6, pp. 1086–1107, 2015.
  7. J.-M. Graïc, L. Finos, V. Vadori, B. Cozzi, R. Luisetto, T. Gerussi, M. Gatto, A. Doria, E. Grisan, L. Corain et al., “Cytoarchitectureal changes in hippocampal subregions of the nzb/w f1 mouse model of lupus,” Brain, Behavior, & Immunity-Health, vol. 32, p. 100662, 2023.
  8. J.-M. Graïc, L. Corain, L. Finos, V. Vadori, E. Grisan, T. Gerussi, K. Orekhova, C. Centelleghe, B. Cozzi, and A. Peruffo, “Age-related changes in the primary auditory cortex of newborn, adults and aging bottlenose dolphins (tursiops truncatus) are located in the upper cortical layers,” Frontiers in Neuroanatomy, vol. 17, p. 1330384, 2024.
  9. M. Á. García-Cabezas, Y. J. John, H. Barbas, and B. Zikopoulos, “Distinction of neurons, glia and endothelial cells in the cerebral cortex: an algorithm based on cytological features,” Frontiers in Neuroanatomy, vol. 10, p. 107, 2016.
  10. J. M. Phillip, K.-S. Han, W.-C. Chen, D. Wirtz, and P.-H. Wu, “A robust unsupervised machine-learning method to quantify the morphological heterogeneity of cells and nuclei,” Nature protocols, vol. 16, no. 2, pp. 754–774, 2021.
  11. Z. Pincus and J. Theriot, “Comparison of quantitative methods for cell-shape analysis,” Journal of microscopy, vol. 227, no. 2, pp. 140–156, 2007.
  12. J. Burgess, J. J. Nirschl, M.-C. Zanellati, A. Lozano, S. Cohen, and S. Yeung-Levy, “Orientation-invariant autoencoders learn robust representations for shape profiling of cells and organelles,” Nature Communications, vol. 15, no. 1, p. 1022, 2024.
  13. C. Stringer, T. Wang, M. Michaelos, and M. Pachitariu, “Cellpose: a generalist algorithm for cellular segmentation,” Nature Methods, vol. 18, no. 1, pp. 100–106, 2021.
  14. E. Upschulte, S. Harmeling, K. Amunts, and T. Dickscheid, “Contour proposal networks for biomedical instance segmentation,” Medical image analysis, vol. 77, p. 102371, 2022.
  15. F. Hörst, M. Rempe, L. Heine, C. Seibold, J. Keyl, G. Baldini, S. Ugurel, J. Siveke, B. Grünwald, J. Egger et al., “Cellvit: Vision transformers for precise cell segmentation and classification,” Medical Image Analysis, vol. 94, p. 103143, 2024.
  16. V. Vadori, J.-M. Graïc, A. Peruffo, G. Vadori, L. Finos, and E. Grisan, “Cisca and cytodark0: a cell instance segmentation and classification method for histo(patho)logical image analyses and a new, open, nissl-stained dataset for brain cytoarchitecture studies,” arXiv e-prints, pp. arXiv–2409, 2024.
  17. M.-A. Bray, S. Singh, H. Han, C. T. Davis, B. Borgeson, C. Hartland, M. Kost-Alimova, S. M. Gustafsdottir, C. C. Gibson, and A. E. Carpenter, “Cell painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes,” Nature protocols, vol. 11, no. 9, pp. 1757–1774, 2016.
  18. M. K. Driscoll, J. L. Albanese, Z.-M. Xiong, M. Mailman, W. Losert, and K. Cao, “Automated image analysis of nuclear shape: what can we learn from a prematurely aged cell?” Aging (Albany NY), vol. 4, no. 2, p. 119, 2012.
  19. F. P. Kuhl and C. R. Giardina, “Elliptic fourier features of a closed contour,” Computer graphics and image processing, vol. 18, no. 3, pp. 236–258, 1982.
  20. J.-P. Antoine, D. Barachea, R. M. Cesar Jr, and L. da Fontoura Costa, “Shape characterization with the wavelet transform,” Signal Processing, vol. 62, no. 3, pp. 265–290, 1997.
  21. R. B. Yadav, N. K. Nishchal, A. K. Gupta, and V. K. Rastogi, “Retrieval and classification of shape-based objects using fourier, generic fourier, and wavelet-fourier descriptors technique: A comparative study,” Optics and Lasers in engineering, vol. 45, no. 6, pp. 695–708, 2007.
  22. T. Chen and C. Guestrin, “Xgboost: A scalable tree boosting system,” in Proceedings of the 22nd Acm Aigkdd International Conference on Knowledge Discovery and Data Mining, 2016, pp. 785–794.
  23. V. Vadori, J.-M. Graïc, A. Peruffo, G. Vadori, L. Finos, and E. Grisan, “Cytodark0,” Sep. 2024. [Online]. Available: https://doi.org/10.5281/zenodo.13694738

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