Calcium imaging and analysis of the mouse hippocampus or neocortex using miniature microendoscopes

Abstract: Calcium imaging using miniscopes are becoming increasingly popular in the neuroscience community with a multitude of microendoscope versions, lens systems and packages for analysis available as open source. Here we describe in detail how to implant lenses and baseplates for the University of California at Los Angeles (UCLA) miniscope V3 and V4 using a Gradient Index (GRIN) lens for the hippocampus or a combination of prism and GRIN lens for recording in the neocortex. Our protocols contain adaptations from several published protocols however the GRIN-relay-Prism lens system protocol is developed in-house and not previously described. This chapter also suggests how lenses may be re-used and how to test the quality of a reused lens. Lastly, we comment on the difference between analysis software and the computational needs for different packages.