The role of liquid-liquid phase separation in regulating enzyme activity

Abstract: Liquid-liquid phase separation is now recognized as a common mechanism for regulating enzyme activity in cells. Insights from studies in cells are complemented by in vitro studies aimed at developing better understanding of mechanisms underlying such control. These mechanisms are often based on the influence of LLPS on the physicochemical properties of the enzyme's environment. Biochemical mechanisms underlying such regulation include the potential for concentrating reactants together, tuning reaction rates, and controlling competing metabolic pathways. LLPS is thus a powerful tool with extensive utilities for the cell, e.g. for consolidating cell survival under stress or rerouting metabolic pathways in response to the energy state of the cell. By examining the evidence of how LLPS affects enzyme catalysis, we can begin to understand emerging concepts and expand our understanding of enzyme catalysis in living cells.