Differential orientation and conformation of Keratinocyte Growth Factor observed at HEMA, HEMA/MMA, and HEMA/MAA hydrogel surfaces developed for wound healing

Abstract: The development of hydrogels for protein delivery requires protein-hydrogel interactions that cause minimal disruption of the protein's biological activity. Biological activity can be influenced by factors such as orientation and conformation. Hydrogels must promote the adsorption of biomolecules onto the surface and the diffusion of biomolecules into the porous network at the surface, while maintaining native protein conformation, keeping the protein in an accessible orientation for receptor binding, and maximizing protein release. We report here the evaluation of (hydroxyethyl)methacrylate (HEMA)-based hydrogel systems for the delivery of keratinocyte growth factor (KGF) to promote re-epithelialization in wound healing. In this work, we characterize two hydrogel blends in addition to HEMA alone, and report how protein orientation, conformation, and protein release is affected. The first blend incorporates methyl methacrylate (MMA), which is known to promote adsorption of protein to its surface due to its hydrophobicity. The second blend incorporates methacrylic acid (MAA), which is known to promote the diffusion of protein into its surface due to its hydrophilicity. We find that KGF at the surface of the HEMA/MMA blend appears to be more orientationally accessible and conformationally active than KGF at the surface of the HEMA/MAA blend. We also report that KGF at the surface of the HEMA/MAA blend becomes conformationally denatured, likely due to hydrogen bonding. While KGF at the surface of these blends can be differentiated by FTIR-ATR spectroscopy and ToF-SIMS in conjunction with PCA, KGF swelling, uptake, and release profiles are indistinguishable. The differences in KGF orientation and conformation between these blends may result in different biological responses in future cell-based experiments.