Compressive fluorescence spectral imaging with a spectrometer

Abstract: We present an efficient approach and principle experiment for compressive sensing (CS) fluorescence spectral imaging. According to the dimension-reduced effect of CS, the spectral and spatial information was simultaneously obtained by using a fiber spectrometer without mechanical scanning. As a method verification, we demonstrated spectral imaging under only two typical wavelengths, but the spectral resolution is up to 1.4nm depended on the fiber spectrometer. The method could obtain 50% light energy from the object, much larger compared with mechanical scanning which detects light of only one point per measurement. The relationship between sampling rate and image quality is also discussed in this study.