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Explain the rationale for attached dormancy at ~1,000-cell chains

Determine the evolutionary or physiological rationale underlying the choice by Escherichia coli multicellular chains to attach to surfaces and halt cell division at approximately 1,000 cells during self-organization.

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Background

During self-organization, E. coli chains attach at lengths of ~200–400 μm (about 1,000 cells) and simultaneously stop moving and dividing. The authors discuss possible explanations, including nutrient access limitations and activation of the stress-response regulator RpoS prior to attachment, but the definitive purpose of this transition remains unresolved.

References

Why E. coli does this is still unclear, though attaching to a rigid and stable surface is a good way for multicellular communities to fix their cell-cell interactions for the long-term.

Multicellular self-organization in Escherichia coli (2503.03001 - Puri et al., 4 Mar 2025) in Section 2.3 Attached dormancy