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Promoter length and regulatory architecture for transgene expression

Ascertain whether a ~1-kilobase upstream promoter region is optimal for driving transgene expression in tardigrades or whether regulatory sequences are uniquely compact, and identify promoter configurations that support robust, tissue-specific expression.

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Background

The TardiVec system uses ~1 kb upstream regions from housekeeping genes to drive expression, but the optimal promoter length and regulatory architecture in tardigrades is unknown.

Clarifying promoter requirements will enhance expression efficiency, reduce variability, and enable reliable pan-neuronal and cell-type-specific labeling.

References

However, it is unclear whether this promoter length is optimal, or if regulatory sequences in tardigrades are uniquely compact.

— The tardigrade as an emerging model organism for systems neuroscience (2501.06606 - Lyons et al., 11 Jan 2025) in Section II. Call to Action: Molecular biology, genomics, and genetic engineering tools, Promoter optimization