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Extent of measurement-error influence on observed N/C ratio fluctuations

Ascertain the extent to which measurement error accounts for the temporal fluctuations observed in experimental time series of the nuclear-to-cell ratio, distinguishing measurement noise from genuine biological variability.

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Background

Based on typical protein counts, the model predicts that N/C ratio fluctuations should be negligible for biologically realistic system sizes. Nonetheless, prior experiments reported time-dependent N/C ratio fluctuations.

The authors explicitly state uncertainty regarding the portion of these observed fluctuations attributable to measurement error, highlighting the need for quantitative separation of experimental noise from biological noise.

References

However, we have observed in our previous experiments that the N/C ratio does fluctuate in time (Figure 7C in ), although it is unclear to what extent these fluctuations are caused by measurement errors.

— Stochastic Gene Expression Model of Nuclear-to-Cell Ratio Homeostasis (2407.19066 - Bai et al., 26 Jul 2024) in Discussion