Implications of cellular chirality in physiology and pathology

Determine the implications of cellular chirality in physiology and pathology by quantifying the contribution of chiral active stresses in circularly confined monolayers of cell lines with known intrinsic chirality bias (e.g., NIH fibroblasts with clockwise bias and C2C12 myoblasts with counterclockwise bias).

Background

The study finds that chiral stresses are negligible in MDCK epithelial monolayers confined to circular patterns and observes no bias between clockwise and counterclockwise rotations. However, other cell lines (e.g., NIH and C2C12) display intrinsic chirality biases when confined to rings or linear stripes.

The authors suggest that measuring the contribution of chiral stress in such chirality-biased lines within circular patterns could shed light on the broader biological implications of cellular chirality, which they explicitly state remains an open question.