Confining photonic nanojet in a microwell on microsphere lens for highly efficient light focusing, signal amplification and quantitative detection

Abstract: Dielectric microspheres or microbeads can squeeze light into the subwavelength scale via photonic nanojet (PNJ) focusing. This enables strong light-matter interactions within its focus and induces extraordinary effects such as enhancements in light emission, signal collection and various other applications. However, critical challenges exist on how to efficiently and precisely loading the studied objects into the desired location of the PNJ focusing, and subsequently measure the field and signal of interests precisely and reliably. Such technique is currently missing. We present for the first time a unique microwell-decorated microsphere lens (u-well lens), with a semi-open microwell sample compartment directly fabricated on top of the microsphere lens. The u-well lens confines PNJ in a semi-open microwell and allows passive trapping of individual micro-object into the PNJ focusing area with high efficiency and spatial accuracy. We demonstrate that individual fluorescent microsphere of different sizes can be readily introduced to the designated location with loading efficiency >70% and generates reproducible enhanced fluorescence signals with standard deviation better than that can be quantitatively measured. A comprehensive analysis on the optical properties of the u-well lens reveals the synergistic effect of field enhancement and collection efficiency on the optical enhancement. We finally employ this special microsphere lens for fluorescent-bead-based biotin concentration analysis. The results suggest a greatly enhanced sensitivity and highly improved detection limit, opening the door for its application in highly sensitive and quantitative detection.