Synthesis of Methotrexate loaded Cerium fluoride nanoparticles with pH sensitive extended release coupled with Hyaluronic acid receptor with plausible theranostic capabilities for preclinical safety studies

Abstract: A key challenge in drug delivery systems is the real time monitoring of delivered drug and subsequent response. Recent advancement in nanotechnology has enabled the design and preclinical implementation of novel drug delivery systems (DDS) with theranostic abilities. Herein, fluorescent cerium fluoride (CeF3) nanoparticles (nps) were synthesized and their surface modified with a coat of polyethylenimine (PEI). Thereafter, Methotrexate was conjugated upon it through glutaraldehyde crosslinking for a pH-sensitive release. This was followed by the addition of a Hyaluronic acid (HA) receptor via 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-hydroxysuccinimide (EDC-NHS) chemistry to achieve a possible active drug targeting system. The obtained drug delivery nano-agent retains and exhibits unique photo-luminescent properties attributed to the nps while exhibiting potential theranostic capabilities.