A detailed model of gene promoter dynamics reveals the entry into productive elongation to be a highly punctual process (2201.13092v1)

Abstract: Gene transcription is a stochastic process that involves thousands of reactions. The first set of these reactions, which happen near a gene promoter, are considered to be the most important in the context of stochastic noise. The most common models of transcription are primarily concerned with the effect of activators/repressors on the overall transcription rate and approximate the basal transcription processes as a one step event. According to such effective models, the Fano factor of mRNA copy distributions is always greater than (super-Poissonian) or equal to 1 (Poissonian), and the only way to go below this limit (sub-Poissonian) is via a negative feedback. It is partly due to this limit that the first stage of transcription is held responsible for most of the stochastic noise in mRNA copy numbers. However, by considering all major reactions that build and drive the basal transcription machinery, from the first protein that binds a promoter to the entrance of the transcription complex (TC) into productive elongation, it is shown that the first two stages of transcription, namely the pre-initiation complex (PIC) formation and the promoter proximal pausing (PPP), is a highly punctual process. In other words, the time between the first and the last step of this process is narrowly distributed, which gives rise to sub-Poissonian distributions for the number of TCs that have entered productive elongation. In fact, having simulated the PIC formation and the PPP via the Gillespie algorithm using 2000 distinct parameter sets and 4 different reaction network topologies, it is shown that only 4.4% give rise to a Fano factor that is > 1 with the upper bound of 1.7, while for 31% of cases the Fano factor is below 0.5, with 0.19 as the lower bound. These results cast doubt on the notion that most of the stochastic noise observed in mRNA distributions always originates at the promoter.